Published: 25 September, 2017 | Volume 1 - Issue 1 | Pages: 075-091
Figure 4:
4: Modulation of MRF genes expression during differentiation. The transcripts encoded by PRDM genes was measured by RT-PCR and qRT-PCR after 24, 48, 72 hours of differentiation induction (see Materials and methods section). The expression level is indicated as fold changes from basal conditions. The PRDM PR and PRDM2tot sets of primers recognize sequences on the region coding PR domain of PRDM genes or on a region common to both PRDM PR plus and minus, respectively. A) Electrophoretic analysis of the fragments obtained by RT-PCR of total mRNA/cDNA extracted from C2C12 cells; B) qRTPCR of total mRNA/cDNA extracted from C2C12 cells. Histograms represent the averages (+/− standard error) from at least three independent experiments, normalized for the expression of the control housekeeping gene GAPDH (# indicates p < 0.05 for each form of PRDM genes versus their untreated control).
Read Full Article HTML DOI: 10.29328/journal.hjbm.1001007 Cite this Article Read Full Article PDF
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