Published: 25 September, 2017 | Volume 1 - Issue 1 | Pages: 075-091
Figure 7:
7: Effect on cell proliferation and survival upon RIZ1 knock down in C2C12 cell line. (A) For the colorimetric MTT assay, C2C12 cells transiently transfected with a plasmid encoding a miRNA against RIZ1 or a not-targeting plasmid (ctrl) were plated at the same density and allowed to grow for 0, 24 or 48 h. MTT was added in the last 2 h, formazan precipitates were dissolved with dimethyl sulfoxide reagent and absorbance read at 570 nm [31]. Values are the mean (±SE) of three analyses from three independent experiments # p < 0.05 vs. control; (B) C2C12 cells transiently transfected with a plasmid encoding a miRNA against RIZ1 or a not-targeting plasmid (ctrl) were cultured in 60-mm dishes for 48 h. Cells were processed following manufacturer’s instruction and finally analyzed by flow cytometer to determine the percentage of cells in the different phases of cell cycle. The data are the mean of three independent experiments performed in triplicate ( = 9) # p < 0.05 vs control.
Read Full Article HTML DOI: 10.29328/journal.hjbm.1001007 Cite this Article Read Full Article PDF
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